 | |
|
| |
 | |
| |
| |
| |
 | |
| |
Real World Test Cases
Below are some test cases taken from commissioning experiments for the mid IR beamline showing real world performance obtainable with synchrotron light.
Figure 1: Diffraction limited map of the cilium in a retinal rod cell. This was a transmission measurement done in D2O.
Figure 2: Linear dichroism measurements of intact rod cells in D2O buffer solution. Top spectrum shows inclination of rhodopsin α-helices: Micro-IR ⟨θ⟩ = 28°, XRD ⟨θ⟩= 24°. Spectrum below shows the lipid acyl chain orientation ν=C-H ⟨θ⟩= 45°, ν CH2 ⟨θ⟩= 61°, with average tilt for an acyl chain of 35°. The blue spectra correspond to measurements parallel to the rod axis, while red is perpendicular.The left picture shows the lipid ester carbonyl orientation νC=O ⟨θ⟩= 70° (67° and 72°).
Figure 3: Diffraction limited maps of 1951 USAF target. Reflection geometry comparing synchrotron and globar sources.
Figure 4: Mapping a 2.7 μm thick PDMA pattern on a silicon wafer. The plot shows the absorption at 1510 cm-1, using a transmission geometry (36X objective and condenser), 3 μm x 3 μm upper aperture, and 1 μm step size.
Figure 5: Mapping of creatine crystals in brain tissue with decreasing confocal aperture size. Reflection geometry. Sample spectra on the right showing results of measuring with two different aperture sizes, as a comparison. Sample courtesy of Kathy Gough, University of Manitoba.